The Ediacaran Period witnessed the first appearance of macroscopic animal life in Earth's history. However, the biogeochemical context for the stratigraphic occurrence of early metazoans is largely uncertain, in part due to the dearth of integrated paleobiological and chemostratigraphic datasets. In this study, a comprehensive geochemical analysis was conducted on the fossiliferous Khatyspyt Formation in Arctic Siberia, in order to gain insights into the Ediacaran paleoenvironments. This study was designed to specifically address the relationship between paleoredox conditions and Ediacaran fossil occurrences in the Khatyspyt Formation. Our data reveal a dramatic shift in pyrite sulfur isotope compositions (δ34Spyrite) from ca. - 20‰ to ca. 55‰, and this shift is intriguingly associated with the first occurrence of Ediacara-type macrofossils at the studied section, suggesting a possible link between seawater redox conditions and distribution of early macroscopic organisms.