New micropaleontological and paleomagnetic data were obtained by studying core samples of Cenozoic continental deposits from two boreholes drilled in the south of Tyumen oblast (Western Siberia). Palynological assemblages in deposits of the Tavda (upper part), Novomikhailovka, Turtas, Abrosimovka, Tobolsk, Smirnovka, and Suzgun formations were described. Deposits of these formations are enriched in spore-pollen assemblages, which can be correlated with assemblages of regional palynozones of the West Siberian Plain. Ostracods were described in Quaternary deposits. On the basis of biostratigraphic and paleomagnetic data, the Late Eocene (Priabonian)-Holocene age of deposits was substantiated. For the first time, beds with dinocysts of genus Pseudokomewuia were identified in deposits of the Turtas Formation (Upper Oligocene) of the Ishim lithofacial area. In total, nine regional magnetozones were distinguished in the paleomagnetic section. On the basis of palynological and paleomagnetic data, sections of two boreholes were correlated, and hiatuses in sedimentation were revealed. A large hiatus is at the Eocene-Oligocene boundary (Western Siberia): the Lower Oligocene Atlym Horizon and Miocene-Pliocene and Eopleistocene sediments are missing.