Semisopochnoi Island is a remote and little-studied volcanic island in the western part of the Aleutian Arc. The existence of several active volcanic centers and a 50007000-year-old large caldera makes this island an important site for volcanic hazard assessment in the Northern Pacific. Based on local seismicity data recorded by six permanent seismic stations, we created the seismic tomography model, including the 3D distributions of Vp, Vs, and Vp/Vs ratios to a depth of 10 km. This model provides the first geophysical insight into the interior structure of Semisopochnoi Island and sheds light on the processes in the magma plumbing system beneath all volcanic centers on the island. At depths of 510 km, we observed a columnar-shaped high Vp/Vs-ratio anomaly below the caldera in the central part of the island, which likely represents the steady magma conduit. This conduit is headed by a prominent high Vp/Vs-ratio anomaly located 35 km directly below the caldera, which represents the magma reservoir feeding Cerberus and other Holocene-aged volcanic centers on Semisopochnoi Island.