The paper presents the first paleontological data (pollen, plant macrofossil, diatom and ostracod) on the sediments of Lake Beloye, which is situated in the forest-steppe zone of Western Siberia. Our study contributes to better understanding of the Middle-Late Holocene regional to local environmental changes. Regionally, we recognized a cold stage of 3.4-2.3 calibrated ka BP and a dry stage of 2.8-1.7 ka BP. The dry stage coincides with the climate-driven lowered level of the lake at 2.6-1.5 ka BP.